Generator

Part:BBa_K2381018:Design

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-23)


light-activated rational split dCas9 with rigid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1420
    Illegal NheI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1420
    Illegal BamHI site found at 4495
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1420
    Illegal AgeI site found at 2357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3155


Design Notes

No.


Source

No.

References